Enzyme Activity Lab Report

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After the reaction has taken place we found out that catalase is not reusable and the hydrogen peroxide is now just water.

The second part of the lab we tested out different substances such as potato, apple, and chicken to see if they contained catalase and how great of a reaction they would have with hydrogen peroxide.

In this lab we studied the catalase found in liver cells.2.

Using forceps and scissors cut a small piece of liver and add it to the test tube. ______________________Recall that a reaction that absorbs heat is endothermic; a reaction that gives off heat is exothermic.

Place 2 ml of hydrogen peroxide in each of 3 clean test tubes and then add each of the three test substances to the tubes.

As you add each test substance, record the reaction rate (0-5) for each tube.1.The theory that catalase can be destroyed was tested in a lab setting using the procedures provided by Mrs. We examined this through the use of Hydrogen Peroxide in its reaction with the catalase. The catalase, or lack thereof, in apples and potatoes was not as readily observable.Also, after superheating and supercooling the liver, we found that the reaction with the Hydrogen Peroxide was diminished. In this lab, we explore the true functions of enzymes.The purpose of this lab was to see how catalase from different hosts reacted in different environments.We learned that the catalase found in chicken liver reacted less when cooled, and not at all when heated.In enzymatic reactions, the molecules at the beginning of the process are called substrates, and the enzyme converts them into different molecules, the products.Almost all processes in a biological cell need enzymes to occur at significant rates.As you add each test substance, record the reaction rate (0-5) for each tube.2.Remove the test tube from the hot water bath, allow it to air cool, then pour out the water. CAUTION: Use a test-tube holder for hot test tubes.Since enzymes are selective for their substrates and speed up only a few reactions from among many possibilities, the set of enzymes made in a cell determines which metabolic pathways occur in that cell. Enzymes work by lowering the activation energy for a reaction, thus dramatically increasing the rate of the reaction.In this lab, we are dealing with the enzyme that is usually found in the cells of many living tissues.

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